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Friday 01 April 2005

An IkappaB-beta COOH terminal region protein is essential for the proliferation of CHO cells under acidic stress.

By: Lao Q, Kuge O, Fukamachi T, Kakegawa T, Saito H, Nishijima M, Kobayashi H.

J Cell Physiol 2005 Apr;203(1):186-92

CHO-K1 cells were able to proliferate and maintain pHi homeostasis at pH 6.3. A novel acidic sensitive mutant, AS-5B, which proliferated at pH 7.4 but failed to either proliferate or maintain pHi homeostasis at pH 6.3, was derived from CHO-K1 using a replica method. The acidic-sensitivity of AS-5B was not due to deficiencies in sodium proton exchangers, HCO3- (co)transporters or H+-ATPases. A cDNA clone encoding a COOH terminal region of IkappaB-beta conferred partial acidic-resistance on AS-5B, and the encoded protein was present in CHO-K1, but was nearly absent from AS-5B. Our data demonstrated that the expression of this small protein was essential for the proliferation of CHO cells under acidic stress. 2004 Wiley-Liss, Inc.

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