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Saturday 01 November 2003

[Inhibitive effect of curcumin and amiloride on the fibrosis of rat hepatic stellate cells induced by oxidative stress]

By: Yang W, Chen H, Jiang Y.

Zhong Yao Cai 2003 Nov;26(11):795-8

OBJECTIVE: To investigate the effects of antioxidant curcumin and Na+/H+ exchanger inhibitor amiloride on the fibrosis of rat hepatic stellate cells (HSC) induced by oxidative stress in vitro. METHODS: HSC were incubated with 0.1 mmol/L ferric nitrilotriacetate complex (FeNTA). MTT colorimetry was used for assaying proliferation of HSC. Cytotoxicity was measured by LDH colorimetry. Collagen type I accummulation in the culture media was measured by ELISA. Intracellular malonildialdehyde (MDA), SOD, GSH and GSH-Px levels in the culture media were measured by respective reagent boxes. RESULTS: HSC incubation with FeNTA resulted in a significant production of intracellular MDA and GSH, associated with decrease of SOD and GSH-PX activity. Exposure of HSC to FeNTA significantly enhanced the number of proliferating HSC and collagen type I levels in the culture medium. All these effects were reversed by the antioxidant curcumin and by the Na+/H+ exchanger inhibitor amiloride. Combining curcumin with amiloride had better effects on the fibrosis of rat hepatic stellate cells induced by oxidative stress than curcumin or amiloride. CONCLUSION: The effects of combination of antifibrosis drugs curcumin and amiloride on different targets of HSC are superior to curcumin or amiloride.

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