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Thursday 14 August 2003

Amiloride inhibition of the proton-translocating NADH-quinone oxidoreductase of mammals and bacteria.

By: Nakamaru-Ogiso E, Seo BB, Yagi T, Matsuno-Yagi A.

FEBS Lett 2003 Aug 14;549(1-3):43-6

The proton-translocating NADH-quinone oxidoreductase in mitochondria (complex I) and bacteria (NDH-1) was shown to be inhibited by amiloride derivatives that are known as specific inhibitors for Na(+)/H(+) exchangers. In bovine submitochondrial particles, the effective concentrations were about the same as those for the Na(+)/H(+) exchangers, whereas in bacterial membranes the inhibitory potencies were lower. These results together with our earlier observation that the amiloride analogues prevent labeling of the ND5 subunit of complex I with a fenpyroximate analogue suggest the involvement of ND5 in H(+) (Na(+)) translocation and no direct involvement of electron carriers in H(+) (Na(+)) translocation.

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