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Saturday 05 August 2006

Effect of terbutaline on alveolar liquid clearance after oleic acid-induced lung injury in rats.

By: Tao J, Yang TD, Li H, DU ZY

Objective: To investigate whether terbutaline affects alveolar liquid clearance after oleic acid-induced lung injury in rats.

Methods:Forty healthy Wistar rats (weighing 250-280 g) were randomly divided into five groups ( n=8 in each group): the normal control group (control group), oleic acid injury group (injury group), terbutaline-treated group (terbutaline group), terbutaline plus amiloride-treated group (terbutaline+amiloride group) and terbutaline plus ouabain-treated group (terbutaline+ouabain group). Acute lung injury model was induced by intravenous oleic acid (0.25 ml/kg body weight). 24 hours later, 1.5 muCi (125) I- labeled 5percent albumin solution (5 ml/kg body weight) was dripped into the lungs through trachea. The alveolar liquid clearance rate, extravascular lung water content, and arterial blood gas were measured 1 hour thereafter. RESULTS: At 24 hours after infusion of oleic acid, the rats developed pulmonary edema and severe hypoxemia, with the alveolar liquid clearance rate decreased by 49.2 percent and the extravascular lung water content elevated by 47.9 percent. Compared with the rats in the injury group, terbutaline (10(-4) mol/L) significantly increased the alveolar liquid clearance rate, decreased the extravascular lung water content and improved hypoxemia. The effect of terbutaline was partly blocked by amiloride and ouabain, which were inhibitors of sodium transport. Terbutaline increased the alveolar liquid clearance rate by 63.7 percent, and amiloride and ouabain reduced the alveolar liquid clearance rate by 54.7 percent and 56.8percent, respectively. CONCLUSIONS: Terbutaline can accelerate alveolar liquid clearance through increasing sodium transport to attenuate pulmonary edema, thus improving gas exchange, which may have therapeutical effect on pulmonary edema after acute lung injury.

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